Work

Amplification of the Tetracycline Resistance Determinant of pAMα1 in Enterococcus faecalis Requires a Site-Specific Recombination Event Involving Relaxase

Year: 2002

Type: article

Abstract: The small multicopy plasmid pAMalpha1 (9.75 kb) encoding tetracycline resistance in Enterococcus faecalis is known to generate tandem repeats of a 4.1-kb segment carrying tet(L) when cells are grown extensively in the presence of tetracycline. Here we show that the initial (rate-limiting) step involves a site-specific recombination event involving plasmid-encoded relaxase activity acting at two recombination sequences (RS1 and RS2) that flank the tet determinant. We also present the complete nucleotide sequence of pAMalpha1.

Source: Journal of Bacteriology

DOI: 10.1128/jb.184.18.5187-5193.2002

Authors: M. Victoria Francia, Don B. Clewell

Institution: University of Michigan

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G3978379768, SEX PHEROMONE INDUCED PLASMID TRANSFER