Work

Long-Range Communication between the Silencers of HMR

Year: 2008

Type: article

Abstract: Gene regulation involves long-range communication between silencers, enhancers, and promoters. In Saccharomyces cerevisiae, silencers flank transcriptionally repressed genes to mediate regional silencing. Silencers recruit the Sir proteins, which then spread along chromatin to encompass the entire silenced domain. In this report we have employed a boundary trap assay, an enhancer activity assay, chromatin immunoprecipitations, and chromosome conformation capture analyses to demonstrate that the two HMR silencer elements are in close proximity and functionally communicate with one another in vivo. We further show that silencing is necessary for these long-range interactions, and we present models for Sir-mediated silencing based upon these results.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.01647-07

Authors: Lourdes Valenzuela, Namrita Dhillon, Rudra N. Dubey, Marc R. Gartenberg, Rohinton T. Kamakaka

Institution: University of California, Santa Cruz

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Binding, Sliding and Function of Cohesin on Sister Chromatids, Chromatin Domain Dynamics, G6824122831, G8443400153, G9000154331