Work

Rapid Pulsed-Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuni

Year: 2001

Type: article

Abstract: We developed a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Campylobacter isolates based on the standardized protocols used by PulseNet laboratories for the subtyping of other food-borne bacterial pathogens. Various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, rapid, and robust. PFGE analysis of Campylobacter isolates can be completed in 24 to 30 h using this protocol, whereas the most widely used current protocols require 3 to 4 days to complete. Comparison of PFGE patterns obtained in six laboratories showed that subtyping results obtained using this protocol are highly reproducible.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.39.5.1889-1894.2001

Authors: Efrain M. Ribot, Collette Fitzgerald, Kristy Kubota, Bala Swaminathan, Timothy J. Barrett

Institution: Centers for Disease Control and Prevention

Language: English

Topic: Salmonella and Campylobacter epidemiology

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

Funder: Centers for Disease Control and Prevention

OpenAlex ID: W2117139877

FWCI: 15.98

Cites: 24

Cited by: 370

Related to: 10