Work

Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Proteins Containing Nonnatural Amino Acids

Year: 2009

Type: article

Abstract: Applied evolution: Regions of the M. jannaschii tyrosyl tRNACUA thought to interact with elongation factor Tu were randomized, and the resulting tRNA libraries were subjected to in vitro evolution. The tRNAs identified resulted in significantly improved yields of proteins containing nonnatural amino acids. In some cases, the degree of improvement varied in an amino acid dependent manner.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.200904035

Authors: Jiantao Guo, Charles E. Melançon, Hyun Soo Lee, Dan Groff, Peter G. Schultz

Institution: Scripps Research Institute

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Evolution of tRNA Synthetases for Incorporation of Novel Backbone Structures, In Vivo Incorporation of Unnatural Amino Acids