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Label-Free Measurements of Reaction Kinetics Using a Droplet-Based Optofluidic Device

Year: 2014
Type: article
Abstract: Label-free measurements of the reaction kinetics of a small sample volume are essential for efficient drug discovery, requiring methods and systems that are rapid, accurate, and cost-effective. Herein, we present an integrated optofluidic system for label-free characterization of reactions in a nanoliter reagent volume. This system contains a droplet-based microfluidic sampling section and an optical fiber-based spectroscopy detection section. By manipulating droplets containing reagents at certain concentrations at different times, quantifiable measurements via absorption spectroscopy can be made in a simple, sensitive, and high-throughput manner. We have demonstrated our system's capability by performing potency (IC50) assays of an inhibitor in a TEM-1 β-lactamase (enzyme) and nitrocefin (substrate) system. This integrated platform can potentially provide an automated, label-free, and low-cost method for many other assays of reaction kinetics.
DOI: 10.1177/2211068214549625
Authors: Zhangming Mao, Feng Guo, Yuliang Xie, Yanhui Zhao, Michael Ian Lapsley, Lin Wang, John D. Mai, Francesco Costanzo, Tony Jun Huang
Language: English
Open Access status: hybrid
Estimated APC paid: $2,070
Funders: National Science Foundation, University of Pennsylvania, Pennsylvania State University, National Institutes of Health, Materials Research Institute, Pennsylvania State University, Materials Research Science and Engineering Center, Harvard University, Division of Materials Research
Awards: G216520254, G3135991130, G5048810679, On-Chip Optofluidic Laser Scanning Confocal Microscope for Early Cancer Detection, G5987211518, MRSEC: Center for Nanoscale Science
OpenAlex ID: W2117686206
FWCI: 0.346
Cites: 53
Cited by: 29
Related to: 10