Work

The Functions of Klarsicht and Nuclear Lamin in Developmentally Regulated Nuclear Migrations of Photoreceptor Cells in theDrosophilaEye

Year: 2003

Type: article

Abstract: Photoreceptor nuclei in the Drosophila eye undergo developmentally regulated migrations. Nuclear migration is known to require the perinuclear protein Klarsicht, but the function of Klarsicht has been obscure. Here, we show that Klarsicht is required for connecting the microtubule organizing center (MTOC) to the nucleus. In addition, in a genetic screen for klarsicht-interacting genes, we identified Lam Dm(0), which encodes nuclear lamin. We find that, like Klarsicht, lamin is required for photoreceptor nuclear migration and for nuclear attachment to the MTOC. Moreover, perinuclear localization of Klarsicht requires lamin. We propose that nuclear migration requires linkage of the MTOC to the nucleus through an interaction between microtubules, Klarsicht, and lamin. The Klarsicht/lamin interaction provides a framework for understanding the mechanistic basis of human laminopathies.

Source: Molecular Biology of the Cell

DOI: 10.1091/mbc.e03-06-0374

Authors: Kristin Patterson, Ari B. Molofsky, Christina Robinson, Shelley Acosta, Courtney Cater, Janice A. Fischer

Institution: The University of Texas at Austin

Language: English

Topic: Nuclear Structure and Function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, University of Texas at Austin, National Institutes of Health, National Eye Institute

Awards: Molecular Genetics of Nuclear Migration During Development, Eye Development: Molecular Genetics of Nuclear Migration, MECHANISM OF NUCLEAR MIGRATION DURING EYE DEVELOPMENT