Year: 2015
Type: article
Abstract: Mature embryos of rice (Oryza sativa L.) varieties Jow Haw was cultured on solid MS (Murashige and Skoog, 1962) and NB (Nitch and Nitch, 1969) medium supplemented with 0.5, 1, 2, 3 and 5 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), 30 g/l sucrose, 1 g/l proline, 100 mg/l casein hydrolysate and 2.6 g/l phytagel. An optimum concentration of 3 mg/l 2,4-D in NB medium was found to be effective for callus induction which induced the biggest size of calli. Transferred the calli to liquid NB medium containing 3 mg/l 2,4-D, 30 g/l sucrose, 1 g/l proline and 100 mg/l casein hydrolysate. Study the growth phases at 0, 3, 6, 9, 12, 15, 18 and 21 days respectively, were determined by measuring fresh and dry weight of the cell. And viability of cell suspensions were determined by the method of fluorescein diacetate. The living cell of cell suspensions showed green fluorescence.
Institution: King Mongkut's Institute of Technology Ladkrabang
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W2118586094