Simultaneous Reconstitution of Multiple Cytomegalovirus‐Specific CD8+Cell Populations with Divergent Functionality in Hematopoietic Stem‐Cell Transplant Recipients
Year: 2005
Type: article
Study design: Observational Study
Abstract: A panel of 7 human cytomegalovirus (CMV) epitope peptides and corresponding major histocompatibility class 1 tetramers was used to evaluate cellular immunity in healthy seropositive donors and in hematopoietic stem-cell transplant recipients. Broad CMV-specific T cell responses to epitopes were found within several CMV polypeptides and were restricted by multiple human leukocyte antigen alleles. Their cytotoxic functionality was evaluated by use of an assay that measures transient surface levels of lysosomal membrane proteins LAMP-1 (CD107a) and LAMP-2 (CD107b) after peptide stimulation. This assay can be combined with tetramer staining of antigen-specific CD8(+) T lymphocytes and has potential as a surrogate marker for cytotoxic function. CD8(+) T lymphocytes specific for epitopes within the pp65 or pp50 gene products exhibited significantly higher functionality, compared with populations recognizing CMV major immediate early-1 epitopes. These functional differences between T lymphocyte populations within the same individual may have implications for protection against CMV.
DOI: 10.1086/428136
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Awards: G15307053, Control of CMV infection post-HCT using attenuated MVA-based CMV subunit vaccine, G3476535037, G5417859779, G5696375705, General Clinical Research Center, G621120552, Hematopoietic Cell Transplantation for Hematologic Malignancies, G675154333, G7051417616, Cancer Center Support Grant
OpenAlex ID: W2118613161