Work

The Drosophila nucleosome remodeling factor NURF is required for Ecdysteroid signaling and metamorphosis

Year: 2005

Type: preprint

Abstract: Drosophila NURF is an ISWI-containing ATP-dependent chromatin remodeling complex that regulates transcription by catalyzing nucleosome sliding. To determine in vivo gene targets of NURF, we performed whole genome expression analysis on mutants lacking the NURF-specific subunit NURF301. Strikingly, a large set of ecdysone-responsive targets is included among several hundred NURF-regulated genes. Null Nurf301 mutants do not undergo larval to pupal metamorphosis, and also enhance dominant-negative mutations in ecdysone receptor. Moreover, purified NURF binds EcR in an ecdysone-dependent manner, suggesting it is a direct effector of nuclear receptor activity. The conservation of NURF in mammals has broad implications for steroid signaling.

Source: Genes & Development

DOI: 10.1101/gad.1342605

Authors: Paul Badenhorst, Hua Xiao, Lucy Cherbas, So Yeon Kwon, Matt Voas, Ilaria Rebay, Peter Cherbas, Carl Wu

Institutions: National Institutes of Health, Indiana University Bloomington, National Cancer Institute, Indiana University, Massachusetts Institute of Technology, University of Birmingham

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, Wellcome Trust, National Institutes of Health, National Cancer Institute

OpenAlex ID: W2118736381

Cites: 41

Cited by: 146

Related to: 10