Novel Protein Antigen (JHP940) from the Genomic Plasticity Region ofHelicobacter pyloriInduces Tumor Necrosis Factor Alpha and Interleukin-8 Secretion by Human Macrophages
Year: 2007
Type: article
Abstract: The plasticity region of the Helicobacter pylori genome comprises strain-specific gene loci. We performed genotyping and functional biology analysis of one such locus (jhp940) that was previously found to be functionally unknown but present in gastric cancer-associated strains from many different countries. We found its geographic prevalence to be independent of cagA presence and disease status. Cloning, expression, and purification of JHP940 revealed a novel, approximately 36-kDa protein in a biologically active form which elicited strong and significant levels of tumor necrosis factor alpha and interleukin-8 in human macrophages. Also, JHP940 was able to induce enhanced translocation of the transcription factor NF-kappaB complex in cultured macrophages. The induction of the proinflammatory cytokines by JHP940, therefore, points to its putative role in chronic gastric inflammation and, possibly, the various other outcomes of H. pylori infection, including gastric cancer.
Source: Journal of Bacteriology
DOI: 10.1128/jb.01309-07
Authors: Mohammed Rizwan, Ayesha Alvi, Niyaz Ahmed
Institution: Centre for DNA Fingerprinting and Diagnostics
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2118909246