Work

Simplified Insertion of Transgenes Onto Balancer Chromosomes via Recombinase-Mediated Cassette Exchange

Year: 2012

Type: article

Abstract: Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE).

Source: G3 Genes Genomes Genetics

DOI: 10.1534/g3.112.002097

Authors: Florence F Sun, Justine E Johnson, Martin P Zeidler, Jack R Bateman

Institutions: Bowdoin College, University of Sheffield

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,709

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Center for Research Resources