Work

An Expanded Set of Fluorogenic Sulfatase Activity Probes

Year: 2014

Type: article

Abstract: Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic probes described here are suitable for various assays and provide a starting point for creating new sulfatase probes with improved selectivity for mycobacterial sulfatases.

Source: ChemBioChem

DOI: 10.1002/cbic.201400032

Authors: Elizabeth L. Smith, Carolyn R. Bertozzi, Kimberly E. Beatty

Institutions: Howard Hughes Medical Institute, University of California, Berkeley, Oregon Health & Science University

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases

Awards: Dynamics of TB and MDR TB transmission in areas with high HIV prevalence, University of California Berkeley Chemistry-Biology Interface Training Program, Sulfatase Activated Fluorescent Probes for In Vivo Diagnostic Imaging of Cancer, Chemical Mycobateriology