Work

Reverse Transcriptase PCR Amplification of Rickettsia typhi from Infected Mammalian Cells and Insect Vectors

Year: 1998

Type: article

Abstract: We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.36.6.1793-1794.1998

Authors: James A. Higgins, Suzana Radulovic, Bruce H. Noden, Jill M. Troyer, Abdu F. Azad

Institution: University of Maryland, Baltimore

Language: English

Topic: Vector-borne infectious diseases

Subfield: Parasitology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: U.S. Public Health Service