Work

Detection of Low-Copy-Number Genomic DNA Sequences in Individual Bacterial Cells by Using Peptide Nucleic Acid-Assisted Rolling-Circle Amplification and Fluorescence In Situ Hybridization

Year: 2007

Type: article

Abstract: An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.02038-06

Authors: Irina Smolina, Charles Lee, Maxim Frank-Kamenetskii

Institutions: Boston University, Brigham and Women's Hospital, Harvard University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

Awards: G4017377196, Fluorescence in situ detection of short DNA sequences