Year: 2009
Type: review
Abstract: Reverse breeding (RB) is a novel plant breeding technique designed to directly produce parental lines for any heterozygous plant, one of the most sought after goals in plant breeding. RB generates perfectly complementing homozygous parental lines through engineered meiosis. The method is based on reducing genetic recombination in the selected heterozygote by eliminating meiotic crossing over. Male or female spores obtained from such plants contain combinations of non-recombinant parental chromosomes which can be cultured in vitro to generate homozygous doubled haploid plants (DHs). From these DHs, complementary parents can be selected and used to reconstitute the heterozygote in perpetuity. Since the fixation of unknown heterozygous genotypes is impossible in traditional plant breeding, RB could fundamentally change future plant breeding. In this review, we discuss various other applications of RB, including breeding per chromosome.
Source: Plant Biotechnology Journal
Authors: Rob Dirks, Kees Van Dun, C. Bastiaan De Snoo, Mark Van Den Berg, Cilia L. C. Lelivelt, William Voermans, Leo Woudenberg, Jack P. C. De Wit, Kees Reinink, Johan W. Schut, Eveline Van Der Zeeuw, Aat Vogelaar, Gerald Freymark, Evert W. Gutteling, Marina N. Keppel, Paul Van Drongelen, Matthieu Kieny, Philippe Ellul, Alisher Touraev, Hong Ma, Hans De Jong, Erik Wijnker
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2120613328