Modified Fibrin Hydrogels stimulate Angiogenesisin vivo: potential Application to increase Perfusion of Ischemic Tissues
Year: 2005
Type: article
Abstract: The lack of angiogenesis in ischemic tissues is a major health problem and many studies aim to explore strategies to locally increase blood perfusion. Our approach is to use covalently modified fibrin-based hydrogels as a matrix that induces endothelial cell survival in vitro and angiogenesis in vivo. Fibrin hydrogels were covalently modified by L1Ig6, a specific receptor for cell survival integrin αvβ3 that is expressed on angiogenic endothelial cells. In addition, L1Ig6-modified matrices were filled with growth factors VEGF-A165 or bFGF. These hydrogels were applied on growing shell-free chicken chorioallantoic membranes (CAMs) and the developing vasculature was found to be increased by ∼50 %. Moreover, the increase in αv-integrin levels in the CAMs underlying the hydrogel implants were investigated and found to be increased by ∼40 % and ∼100 %, respectively, after CAM stimulation with L1Ig6 alone or in combination with growth factors VEGF-A165 and bFGF. Therefore, modified fibrin hydrogels provide an interesting way to design an implant that can be introduced at the site of ischemia, and provides a scaffold and release system for growth factors that induce specific tissue responses.
Authors: H. Hall, J. A. Hubbell
Language: German
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2120734066