Work

Cell viability and DNA denaturation measurements by two-photon fluorescence excitation in CW Al:GaAs diode laser optical traps

Year: 1999

Type: article

Abstract: Cell viability and DNA denaturation are measured through two-photon fluorescence excitation using a single diode laser beam as the trapping and exciting source simultaneously. Two-photon fluorescence emission spectra are presented for CHO cells and T lymphocytes loaded with various fluorescent probes. This single beam method is demonstrated to be a safe tool to monitor the viability of optically trapped cells, even under intense 809 nm diode laser illumination (∼106 W/cm2). The dynamics of cellular necrosis is monitored by adding ethanol to the cell suspension during trapping. Thermal damage to heat-treated samples is assessed by recording shifts in the emission spectra from trapped cells loaded with the nucleic acid probe, acridine orange. © 1999 Society of Photo-Optical Instrumentation Engineers.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.429948

Authors: Zhanxiang Zhang, Gregory J. Sonek, Xunbin Wei, Chung-Ho Sun, Michael W. Berns, Bruce J. Tromberg

Institutions: Physical Optics Corporation, Beckman Laser Institute and Medical Clinic, University of California, Irvine

Language: English

Topic: Orbital Angular Momentum in Optics

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: bronze

Funders: U.S. Department of Energy, National Institutes of Health, Office of Naval Research, National Center for Research Resources

Awards: NEW GRANT - A CENTER OF EXCELLENCE FOR THE MEDICAL APPLICATION OF LASERS, G8876996369, G9000199918