A More Sensitive and Specific Histochemical Peroxidase Stain for the Localization of Cellular Antigen by the Enzyme-antibody Conjugate Method
Year: 1974
Type: article
Abstract: In an attempt to increase the sensitivity and specificity of immunohistochemical procedures using peroxidase-labeled antibody, we have modified the technique. This modification takes advantage of the pH optimum of horseradish peroxidase. The best buffer salts, hydrogen peroxide concentration, time of staining, fixation after incubation with labeled antibody and counterstains were determined empirically. Compounds similar to the substrate are known to undergo photooxidation, and specificity was enhanced by keeping the solutions in the dark.
DOI: 10.1177/22.12.1135
Authors: E. EARL WEIR, THOMAS G. PRETLOW, ANNETTE PITTS, EDWIN E. WILLIAMS
Institution: University of Alabama at Birmingham
Language: English
Subfield: Anatomy
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2121041476