Work

Microscopic Imaging and Spectroscopy with Scattered Light

Year: 2010

Type: review

Abstract: Optical contrast based on elastic scattering interactions between light and matter can be used to probe cellular structure, cellular dynamics, and image tissue architecture. The quantitative nature and high sensitivity of light scattering signals to subtle alterations in tissue morphology, as well as the ability to visualize unstained tissue in vivo, has recently generated significant interest in optical-scatter-based biosensing and imaging. Here we review the fundamental methodologies used to acquire and interpret optical scatter data. We report on recent findings in this field and present current advances in optical scatter techniques and computational methods. Cellular and tissue data enabled by current advances in optical scatter spectroscopy and imaging stand to impact a variety of biomedical applications including clinical tissue diagnosis, in vivo imaging, drug discovery, and basic cell biology.

Source: Annual Review of Biomedical Engineering

DOI: 10.1146/annurev-bioeng-061008-124811

Authors: Nada N. Boustany, Stephen A. Boppart, Vadim Backman

Institutions: Rutgers, The State University of New Jersey, University of Illinois Urbana-Champaign, Northwestern University

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering

Awards: G2362271019, G3342837969, G6885688680, Novel Biophotonics Methodology for Colon Cancer Screening