Work

Analysis of The Primary Structure Of Chromatin with Next-Generation Sequencing

Year: 2010

Type: article

Abstract: The recent development of next-generation sequencing technology has enabled significant progress in chromatin structure analysis. Here, we review the experimental and bioinformatic approaches to studying nucleosome positioning and histone modification profiles on a genome scale using this technology. These studies advanced our knowledge of the nucleosome positioning patterns of both epigenetically modified and bulk nucleosomes and elucidated the role of such patterns in regulation of gene expression. The identification and analysis of large sets of nucleosome-bound DNA sequences allowed better understanding of the rules that govern nucleosome positioning in organisms of various complexity. We also discuss the existing challenges and prospects of using next-generation sequencing for nucleosome positioning analysis and outline the importance of such studies for the entire chromatin structure field.

Source: Epigenomics

DOI: 10.2217/epi.09.48

Authors: Michael Y Tolstorukov, Peter V Kharchenko, Peter J Park

Institutions: Brigham and Women's Hospital, Harvard University, Boston Children's Hospital

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Human Genome Research Institute

Award: Genome-Wide Mapping of Chromosomal Proteins in Drosophilia