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Mutations in the aph(2")-Ic Gene Are Responsible for Increased Levels of Aminoglycoside Resistance

Year: 2002

Type: article

Abstract: Random PCR mutagenesis of the enterococcal aph(2")-Ic gene followed by selection for mutant enzymes that confer enhanced levels of aminoglycoside resistance resulted in mutants of APH(2")-Ic with His-258-Leu and Phe-108-Leu substitutions, all of which conferred rises in the MICs of several aminoglycosides. The mutated residues are located outside conserved regions of aminoglycoside phosphotransferases.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.46.10.3253-3256.2002

Authors: Hae Kyung Lee, Sergei B. Vakulenko, Don B. Clewell, Stephen A. Lerner, Joseph W. Chow

Institutions: Wayne State University, University of Michigan, John D. Dingell VA Medical Center

Language: English

Topic: Antibiotic Resistance in Bacteria

Subfield: Molecular Medicine

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2121259592

FWCI: 0.297

Cites: 19

Cited by: 5

Related to: 10