Work

In‐cell protein NMR and protein leakage

Year: 2010

Type: article

Abstract: In-cell nuclear magnetic resonance spectroscopy is a tool for studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are observed in the liquid surrounding the cells. Here, we examine the expression of four proteins in Escherichia coli. We describe the controls that should be used for in-cell NMR experiments and show that leakage is likely when the protein being studied exceeds ∼20% of the total cellular protein.

Source: Proteins Structure Function and Bioinformatics

DOI: 10.1002/prot.22906

Authors: Christopher O. Barnes, Gary J. Pielak

Institutions: University of North Carolina at Chapel Hill, UNC Lineberger Comprehensive Cancer Center

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health

Awards: NIH Director's Pioneer Award, G5504187562