Pollen viability and germination in Jatropha ribifolia and Jatropha mollissima (Euphorbiaceae): Species with potential for biofuel production
Year: 2011
Type: article
Abstract: The aim of this work is to assess pollen viability using the staining technique and in vitro germination with different concentrations of sucrose in Jatropha ribifolia and Jatropha mollissima , contributing to the knowledge of the reproductive biology and subsidizing their conservation, management and utilization. Pollen viability was measured by dye method. Acetocarmine, acetic orcein and cotton-blue stain were used. The culture medium for pollen germination was solidificated by the addition of 1% agar combined with 0 (control), 10, 20, 30 and 40% of sucrose. The data were submitted to analysis of variance at 5% probability. All dyes used in this experiment allowed easy differentiation between fertile and non fertile pollen. The rate of formation of pollen tubes was higher in medium with 10% of sucrose for both species because the trend is that the sucrose concentration increases the supply of carbon, changes the osmotic potential and inhibits the formation of pollen tube in vitro .
Source: AFRICAN JOURNAL OF BIOTECHNOLOGY
Language: English
Topic: Plant Reproductive Biology
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2121435092