Work

Pleiotrophin Signaling Is Necessary for Hematopoietic Stem Cell Self Renewal and Is Regulated by the Bone Marrow Microenvironment

Year: 2011

Type: article

Abstract: We recently demonstrated that treatment of murine and human HSCs with the heparin binding growth factor, pleiotrophin (PTN), was sufficient to induce self-renewal of murine and human HSCs in culture (Himburg, Nat Med, 2010). In order to determine if PTN signaling is necessary for HSC self renewal and normal hematopoiesis in vivo, we examined mice bearing a constitutive deletion of PTN (PTN −/− mice) (Jackson Laboratory). PTN −/− mice demonstrated no significant differences in total bone marrow (BM) cells or BM colony forming cells (CFCs), but had significantly decreased bone marrow CD34(-)c-kit(+)sca-1(+)lin(-) (34-KSL) cells (0.007% vs.

Source: Transplantation and Cellular Therapy

DOI: 10.1016/j.bbmt.2010.12.002