Accurate transcription initiation of Vicia faba rDNA in a whole cell extract from embryonic axes
Year: 1993
Type: article
Abstract: Summary An in vitro assay system is presented for analyses of transcription of the Vicia faba rRNA gene. The system consists of a whole cell extract from the embryonic axes of V. faba, substrates and exogenously added rDNA fragments. Specific initiation at the promoter was determined by a size analysis of the transcripts synthesized from DNA templates of various molecular sizes. This transcription was dependent on RNA polymerase I, since the reaction mixtures contain α‐amanitin. Furthermore, the primer extension experiment showed that at least a part of each transcript started at the same nucleotide, which was previously determined by in vivo S1 analysis. Therefore, it is suggested that accurate transcription of the rRNA gene occurred in this system.
Source: The Plant Journal
Authors: Junko Yamashita, Tadaka Nakajima, Shigeyuki Tanifuji, Atsushi Kato
Institution: Hokkaido University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2122426439