Year: 2005
Type: other
Abstract: UV-inducedd DNA damage causes cells to repress RNA synthesis and to initiate nucleotide excisionn repair (NER).NER and transcription are intimately linked processes.Evidence has beenn presented that, in addition to damaged genes, undamaged loci are transcriptionally inhibited..We investigated whether RNA synthesis from undamaged genes is affected by thee presence of UV damage elsewhere in the same nucleus, using a novel technique to UV irradiatee only part of a nucleus.We show that the basal transcript ion/repair factor TFI1H is recruitedd to the damaged nuclear area, partially depleting the undamaged nuclear area.Remarkably,, this sequestration has no effect on RNA synthesis.This result was obtained forr cells that are able to carry out NER and for cells deficient in NER.We conclude that crosss talk between NER and transcription occurs only over short distances in nuclei of livingg cells.3..
Author: Martijn J. Moné
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2122475528