Work

Phosphatidylinositol-4-phosphate 5-Kinase-1β Is Essential for Epidermal Growth Factor Receptor-mediated Endocytosis

Year: 2001

Type: article

Abstract: Phosphatidylinositol-4,5-bisphosphate (PIP(2)) is known to play an important role in signal transduction and membrane trafficking. We show that one enzyme responsible for PIP(2) production, phosphatidylinositol-4-phosphate 5-kinase type 1beta (PIPKbeta), is essential for epidermal growth factor receptor (EGFR)-mediated endocytosis. Expression of murine PIPKbeta in NR6 cells expressing EGFR strikingly increased receptor internalization. Moreover, the kinase was shown to form an immunoprecipitable complex with EGFR. Expression of either a truncated kinase or a kinase dead mutant inhibited EGFR endocytosis and also blocked the membrane recruitment of PIPKbeta and both clathrin light chain and dynamin. Our results delineate a novel mechanism by which PIPKbeta regulates receptor-mediated endocytosis and receptor tyrosine kinase membrane traffic.

Source: Journal of Biological Chemistry

DOI: 10.1074/jbc.c100490200

Authors: M. Alejandro Barbieri, Colin M. Heath, Elizabeth M. Peters, Alan Wells, J. Nathan Davis, Philip D. Stahl

Institutions: Washington University in St. Louis, Louisiana State University Health Sciences Center Shreveport, University of Pittsburgh

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430