Work

The YbgC protein encoded by the ybgC gene of the tol‐pal gene cluster of Haemophilus influenzae catalyzes acyl‐coenzyme A thioester hydrolysis

Year: 2002

Type: article

Abstract: This paper examines the catalytic function of the protein YbgC, encoded by the ybgC gene of the tol-pal gene cluster in Haemophilus influenzae. The YbgC protein, a homologue of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-coenzyme A thioesterase, conserves the active site Asp residue associated with thioesterase activity. The H. influenzae ybgC gene was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and tested for thioesterase activity towards acyl-CoA and acyl-N-acetylcysteamine thioesters. The YbgC protein catalyzes the hydrolysis of short chain aliphatic acyl-CoA thioesters, while the D18N YbgC mutant protein (prepared to serve as a control) does not.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(02)02533-4

Authors: Zhihao Zhuang, Feng Song, Brian M Martin, Debra Dunaway-Mariano

Institutions: University of New Mexico, National Institutes of Health, National Institute of Mental Health

Language: English

Topic: Lipid metabolism and biosynthesis

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Enzymes: Structure, Mechanism, Function and Inhibition, G470414181