Work

Single‐Molecule Experiments in Synthetic Biology: An Approach to the Affinity Ranking of DNA‐Binding Peptides

Year: 2005

Type: article

Abstract: Peptide–DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.200500152

Authors: Rainer Eckel, Sven David Wilking, Anke Becker, Norbert Sewald, Robert Ros, Dario Anselmetti

Institution: Bielefeld University

Language: English

Topic: Force Microscopy Techniques and Applications

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2122559962

FWCI: 3.266

Cites: 35

Cited by: 53

Related to: 10