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Two Members of the Tcf Family Implicated in Wnt/β-Catenin Signaling during Embryogenesis in the Mouse

Year: 1998
Type: article
Abstract: Tcf transcription factors interact with beta-catenin and Armadillo to mediate Wnt/Wingless signaling. We now report the characterization of genes encoding two murine members of the Tcf family, mTcf-3 and mTcf-4. mTcf-3 mRNA is ubiquitously present in embryonic day 6.5 (E6.5) mouse embryos but gradually disappears over the next 3 to 4 days. mTcf-4 expression occurs first at E10.5 and is restricted to di- and mesencephalon and the intestinal epithelium during embryogenesis. The mTcf-3 and mTcf-4 proteins bind a canonical Tcf DNA motif and can complex with the transcriptional coactivator beta-catenin. Overexpression of Wnt-1 in a mammary epithelial cell line leads to the formation of a nuclear complex between beta-catenin and Tcf proteins and to Tcf reporter gene transcription. These data demonstrate a direct link between Wnt stimulation and beta-catenin/Tcf transcriptional activation and imply a role for mTcf-3 and -4 in early Wnt-driven developmental decisions in the mouse embryo.
DOI: 10.1128/mcb.18.3.1248
Authors: Vladimir Korinek, Nick Barker, Karl Willert, Miranda Molenaar, Jeroen Roose, Gerry Wagenaar, Marry Markman, Wout Lamers, Olivier Destree, Hans Clevers
Institutions: University Medical Center Utrecht, Howard Hughes Medical Institute, Stanford Medicine, Stanford University, Hubrecht Institute for Developmental Biology and Stem Cell Research, University of Amsterdam
Language: English
Open Access status: green
OpenAlex ID: W2122591546
FWCI: 8.328
Cites: 48
Cited by: 325
Related to: 10