Biomass production of Lactobacillus plantarum LP02 isolated from infant feces with potential cholesterol- lowering ability
Year: 2011
Type: article
Abstract: The potentially hypocholesterolemic strain, designated PL02, of Lactobacillus plantarum , was isolated from infant feces. The aim of this study was to characterize and to cultivate this isolate for biomass production in a 5 L fermentor by batch or fed-batch fermentation. A modified medium composition without peptone was used to produce more viable cells with a 150 rpm agitation speed and 0.5 vvm of aeration during incubation. A cell concentration of 2.2 g dry cell weight (DCW) per liter in fermented broth was reached in a 5 L fermentor after the glucose was consumed completely during batch fermentation. In addition, biomass was significantly improved at 28 h of fed-batch fermentation (9.45 g DCW/l) over a constant feeding rate of 20 ml/h of feeding solution since the glucose was consumed at batch process. A much shorter fermentation time (15 h) and greater biomass (10.12 g DCW/l) was obtained by using multistep feeding rates to maintain glucose concentration of 1 to 5 g/l during fedbatch process. The biomass of L. plantarum PL02 produced by fed-batch fermentation was greater than that achieved by batch fermentation and the fed-batch method might also be suitable for other lactic acid bacteria fermentation. Key words: Lactobacillus plantarum, fermentation, fed-batch fermentation, cholesterol
Source: AFRICAN JOURNAL OF BIOTECHNOLOGY
Institution: Hungkuang University
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2122638840