Year: 2007
Type: article
Abstract: We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.
Authors: Kazuhiro Chiba, Yasutaka Hoshino, Keiko Ishino, Takahisa Kogure, Yuzuru Mikami, Yoshimasa Uehara, Jun Ishikawa
Institutions: National Institute of Infectious Diseases, Chiba University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4391337552