Work

Expression of three human β‐adrenergic‐receptor subtypes in transfected chinese hamster ovary cells

Year: 1991

Type: article

Abstract: The genes coding for three pharmacologically distinct subtypes of human beta-adrenergic receptors (beta 1 AR, beta 2 AR and beta 3 AR) were transfected for expression in Chinese hamster ovary (CHO) cells. Stable cell lines expressing each receptor were analyzed by ligand binding, adenylate cyclase activation and photoaffinity labeling, and compared to beta AR subtypes observed in previously described tissues, primary cultures and transfected cell lines. Each of the three receptor subtypes displayed saturable [125I]iodocyanopindolol-binding activity. They showed the characteristic rank order of potencies for five agonists, determined by measuring the accumulation of intracellular cAMP. These recombinant cell lines express a homogeneous population of receptors and display the known pharmacological properties of beta 1 AR and beta 2 AR, in human tissues. It is therefore likely that the pattern of ligand binding and adenylate cyclase activation, mediated by the new beta 3 AR in CHO cells, also reflects the yet-undetermined pharmacological profile in humans.

Source: European Journal of Biochemistry

DOI: 10.1111/j.1432-1033.1991.tb15824.x

Authors: Keri M. TATE, Marie‐M. BRIEND‐SUTREN, Laurent J. EMORINE, Colette DELAVIER‐KLUTCHKO, Stefano MARULLO, A. Donny STROSBERG

Institutions: Université Paris Diderot, Centre National de la Recherche Scientifique, Institut Cochin

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Centre National de la Recherche Scientifique