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Histone chaperone ASF1 cooperates with the Brahma chromatin-remodelling machinery

Year: 2002
Type: preprint
Abstract: De novo chromatin assembly into regularly spaced nucleosomal arrays is essential for eukaryotic genome maintenance and inheritance. The Anti-Silencing Function 1 protein (ASF1) has been shown to be a histone chaperone, participating in DNA-replication-coupled nucleosome assembly. We show that mutations in the Drosophila asf1 gene derepress silencing at heterochromatin and that the ASF1 protein has a cell cycle-specific nuclear and cytoplasmic localization. Furthermore, using both genetic and biochemical methods, we demonstrate that ASF1 interacts with the Brahma (SWI/SNF) chromatin-remodelling complex. These findings suggest that ASF1 plays a crucial role in both chromatin assembly and SWI/SNF-mediated chromatin remodelling.
DOI: 10.1101/gad.231202
Authors: Yuri M. Moshkin, Jennifer A. Armstrong, Robert K. Maeda, John W. Tamkun, Peter Verrijzer, James A. Kennison, Francois Karch
Institutions: University of Geneva, National Institutes of Health, University of California, Santa Cruz, Leiden University Medical Center, Medical Genetics Center, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Language: English
Open Access status: bronze
OpenAlex ID: W2123533005
Cites: 32
Cited by: 111
Related to: 10