Year: 1967
Type: article
Abstract: A method is described for preparing, by filtration on Millipore filters, very thin (about 10 micro) pellicles of packed particles. These pellicles can be embedded in Epon for electron microscopic examination. They are also suitable for cytochemical assays. The method was used with various particulate fractions from rat liver. Its main advantages over the usual centrifugal packing techniques are that it produces heterogeneity solely in the direction perpendicular to the surface of the pellicle and that sections covering the whole depth of the pellicle can be photographed in a single field. It thus answers the essential criterion of random sampling and can be used for accurate quantitative evaluations.
Source: The Journal of Cell Biology
DOI: 10.1083/jcb.32.1.181
Institutions: UCLouvain, KU Leuven
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: green
Funder: U.S. Public Health Service
OpenAlex ID: W2124536575