Work

Evaluation of the One-Step Multiplex Real-Time Reverse Transcription-PCR ProFlu-1 Assay for Detection of Influenza A and Influenza B Viruses and Respiratory Syncytial Viruses in Children

Year: 2007

Type: article

Abstract: We evaluated the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for the detection of influenza A and influenza B viruses and respiratory syncytial viruses from 353 pediatric nasopharyngeal aspirates. As assessed by comparison with the results of immunofluorescence testing and cell culture, the specificity and the sensitivity of the ProFlu-1 assay ranged from 97% to 100%. In addition, the ProFlu-1 assay amplified 9% of samples not detected by conventional methods.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.00959-07

Authors: Jérôme LeGoff, Rachid Kara, Florence Moulin, Ali Si-Mohamed, Anne Krivine, Laurent Bélec, Pierre Lebon

Institutions: Hôpital Européen Georges-Pompidou, Hôpital Saint-Louis, Université Paris Descartes, Hôpital Cochin

Language: English

Topic: Influenza Virus Research Studies

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2124682865

FWCI: 4.867

Cites: 16

Cited by: 63

Related to: 10