Work

Targeting the site of RNA-RNA recombination in brome mosaic virus with antisense sequences.

Year: 1993

Type: article

Abstract: It has been postulated that local hybridizations between viral RNAs can mediate recombination in brome mosaic virus (BMV) and in poliovirus. To test this model, a 3' fragment of BMV RNA1 was inserted into the 3' noncoding sequence of BMV RNA3 in an antisense orientation. This resulted in high-frequency nonhomologous crossovers at or near the hybridized region. Insertion of the same RNA1 fragment in a positive-sense orientation did not promote recombination. Modification of the antisense insert by deletion of 3' portions did not affect the sites of crossover. However, modification of the 5' portion shifted the crossovers toward the central part of the heteroduplex region. Our results provide experimental evidence that recombinant crosses can be primed by hybridization between viral RNA molecules.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.90.14.6390

Authors: P D Nagy, J J Bujarski

Institution: Northern Illinois University

Language: English

Topic: Plant Virus Research Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Awards: G2123276099, RECOMBINATION IN THE MULTIPARTITE RNA VIRUSES