A doubly responsive probe for the detection of Cys4-tagged proteins
Year: 2015
Type: article
Abstract: Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys-Cys-X-X-Cys-Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized (129)Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the (129)Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.
Source: Chemical Communications
DOI: 10.1039/c5cc04721h
Institutions: Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Laboratoire d'Excellence en Recherche sur le Médicament et l'Innovation Thérapeutique, Inserm, Hôpital Saint-Louis, Génétique des Virus et Pathogénèse des Maladies Virales, École Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Laboratoire de Chimie, CEA Paris-Saclay - Etablissement de Saclay, Institut Rayonnement-Matière de Saclay, CEA Paris-Saclay, Laboratoire Structure et Dynamique par Résonance Magnétique, Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Énergie
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2125716416