Analysis of the real EADGENE data set: Multivariate approaches and post analysis (Open Access publication)
Year: 2007
Type: article
Abstract: The aim of this paper was to describe, and when possible compare, the multivariate methods used by the participants in the EADGENE WP1.4 workshop. The first approach was for class discovery and class prediction using evidence from the data at hand. Several teams used hierarchical clustering (HC) or principal component analysis (PCA) to identify groups of differentially expressed genes with a similar expression pattern over time points and infective agent (E. coli or S. aureus). The main result from these analyses was that HC and PCA were able to separate tissue samples taken at 24 h following E. coli infection from the other samples. The second approach identified groups of differentially co-expressed genes, by identifying clusters of genes highly correlated when animals were infected with E. coli but not correlated more than expected by chance when the infective pathogen was S. aureus. The third approach looked at differential expression of predefined gene sets. Gene sets were defined based on information retrieved from biological databases such as Gene Ontology. Based on these annotation sources the teams used either the GlobalTest or the Fisher exact test to identify differentially expressed gene sets. The main result from these analyses was that gene sets involved in immune defence responses were differentially expressed.
Source: Genetics Selection Evolution
Authors: Peter Sørensen, Agnès Bonnet, Bart Buitenhuis, Rodrigue Closset, Sébastien Déjean, Céline Delmas, Mylène Duval, Liz Glass, Jakob Hedegaard, Henrik Hornshøj, Ina Hulsegge, Florence Jaffrézic, Kirsty Jensen, Li Jiang, Dirk-Jan de Koning, Kim-Anh Lê Cao, Haisheng Nie, Wolfram Petzl, Marco H Pool, Christèle Robert-Granié, Magali San Cristobal, Mogens Sandø Lund, Evert M van Schothorst, Hans-Joachim Schuberth, Hans-Martin Seyfert, Gwenola Tosser-Klopp, David Waddington, Michael Watson, Wei Yang, Holm Zerbe
Institutions: Aarhus University, Laboratoire de Génétique Cellulaire, University of Liège, Université Toulouse III - Paul Sabatier, Université de Toulouse, Institut de Mathématiques de Toulouse, Institut National de la Recherche Agronomique, Roslin Institute, Wageningen University & Research, Ludwig-Maximilians-Universität München, University of Veterinary Medicine Hannover, Foundation, Research Institute for Farm Animal Biology (FBN), The Pirbright Institute
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $2,390
OpenAlex ID: W2126759156