Work

The AlkB Domain of Mammalian ABH8 Catalyzes Hydroxylation of 5‐Methoxycarbonylmethyluridine at the Wobble Position of tRNA

Year: 2010

Type: article

Abstract: Family ties: The AlkB family of nonheme iron, α-ketoglutarate (αKG)-dependent dioxygenases is involved in biological processes such as DNA/RNA repair and obesity. The AlkB domain of ABH8 is shown to catalyze the hydroxylation of a modified uridine (mcm5U) into (S)-mchm5U at the wobble position of the anticodon stem–loop in tRNA (see scheme), thereby suggesting that this protein is a tRNA hypermodification enzyme.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201001242

Authors: Ye Fu, Qing Dai, Wen Zhang, Jin Ren, Tao Pan, Chuan He

Institution: University of Chicago

Language: English

Topic: RNA modifications and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: U.S. Public Health Service, National Institute of General Medical Sciences

Awards: G1874480059, G2415896416, Direct Oxidative Nucleic Acids Demethylation, Discovering the epigenetics of RNA modifications, G6701925027