Work

Real-Time Detection of Virus Particles and Viral Protein Expression with Two-Color Nanoparticle Probes

Year: 2005

Type: article

Abstract: Respiratory syncytial virus (RSV) mediates serious lower respiratory tract illness in infants and young children and is a significant pathogen of the elderly and immune compromised. Rapid and sensitive RSV diagnosis is important to infection control and efforts to develop antiviral drugs. Current RSV detection methods are limited by sensitivity and/or time required for detection. In this study, we show that antibody-conjugated nanoparticles rapidly and sensitively detect RSV and estimate relative levels of surface protein expression. A major development is use of dual-color quantum dots or fluorescence energy transfer nanobeads that can be simultaneously excited with a single light source.

Source: Journal of Virology

DOI: 10.1128/jvi.79.13.8625-8628.2005

Authors: Amit Agrawal, Ralph A. Tripp, Larry J. Anderson, Shuming Nie

Institutions: Georgia Institute of Technology, Emory University, University of Georgia, Centers for Disease Control and Prevention, National Center for Infectious Diseases

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green