Work

Epitope Tagging Genomic DNA Using a CD-Tagging Tn10 Minitransposon

Year: 2002

Type: article

Abstract: Here, we describe an efficient system for epitope tagging cloned genes by CD tagging using a mini-Tn10 transposon delivery vector. The system was tested against a lambdaFIX genomic clone of the human nucleolin gene. Transfection of HeLa cells with the tagged gene led to the expression of both the appropriately spliced tagged transcript and the appropriately localized tagged protein.

Source: BioTechniques

DOI: 10.2144/02322rr04

Authors: C.A. Telmer, P.B. Berget, B. Ballou, R.F. Murphy, J.W. Jarvik

Institution: Carnegie Mellon University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funders: National Science Foundation, National Institutes of Health, National Cancer Institute