Year: 2009
Type: article
Abstract: An over-expressed L-ldh gene derivative of Escherichia coli BAD-ldh was developed. L-ldh gene from Enterococcus facelis KK1 consisted of an open reading frame of 954 bp encoding 316 amino acids. L-ldh gene was cloned into pBAD vector and transformed into E.coli SZ85 by electroporation. SDS-page and western blotting method confirmed the presence of recombinant L-LDH enzyme with the approximate size of 40kD. The activity of L-lactate dehydrogenase was achieved at 170 U ml¯¹. E.coli BAD85 was found to produce 0.62 g l¯¹ of lactic acid from 1 g 1¯¹ of fructose in 24 h. L-ldh gene from was successfully transformed into E.coli SZ85 with the maximum production of L-lactic acid at 0.62 g l¯¹.
Authors: Mulok, Tengku Elida Tengku Zainal, 21814, Chong, Mei-Ling, 21815, 白井, 義人, 8065, Shirai, Yoshihito, 50175395, シライ, ヨシヒト, 7202788909, Rahim, Raha Abdul, 21817, Hassan, Mohd Ali, 21818
Institutions: Universiti Putra Malaysia, Kyushu Institute of Technology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2127985679