Work

A Cryptic Rab1-binding Site in the p115 TetheringProtein

Year: 2005

Type: article

Abstract: Small GTPases and coiled-coil proteins of the golgin family help to tether COPI vesicles to Golgi membranes. At the cis-side of the Golgi, the Rab1 GTPase binds directly to each of three coiled-coil proteins: p115, GM130, and as now shown, Giantin. Rab1 binds to a coiled-coil region within the tail domain of p115 and this binding is inhibited by the C-terminal, acidic domain of p115. Furthermore, GM130 and Giantin bind to the acidic domain of p115 and stimulate p115 binding to Rab1, suggesting that p115 binding to Rab1 is regulated. Regulation of this interaction by proteins such as GM130 and Giantin may control the membrane recruitment of p115 by Rab1.

Source: Journal of Biological Chemistry

DOI: 10.1074/jbc.m503925200

Authors: Matthew Beard, Ayano Satoh, James Shorter, Graham Warren

Institutions: Columbia University, Yale University, Ludwig Cancer Research, Whitehead Institute for Biomedical Research

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W2128352578

FWCI: 2.439

Cites: 59

Cited by: 84

Related to: 10