Measurement of tissue scattering properties using multi-diameter single fiber reflectance spectroscopy: in silico sensitivity analysis
Year: 2011
Type: article
Abstract: Multiple diameter single fiber reflectance (MDSFR) measurements of turbid media can be used to determine the reduced scattering coefficient (μ'(s)) and a parameter that characterizes the phase function (γ). The MDSFR method utilizes a semi-empirical model that expresses the collected single fiber reflectance intensity as a function of fiber diameter (d(fiber)), μ'(s), and γ. This study investigated the sensitivity of the MDSFR estimates of μ'(s) and γ to the choice of fiber diameters and spectral information incorporated into the fitting procedure. The fit algorithm was tested using Monte Carlo simulations of single fiber reflectance intensities that investigated biologically relevant ranges of scattering properties (μ'(s) ∈ [0.4 - 4]mm(-1)) and phase functions (γ ∈ [1.4 - 1.9]) and for multiple fiber diameters (d(fiber) ∈ [0.2 - 1.5] mm). MDSFR analysis yielded accurate estimates of μ'(s) and γ over the wide range of scattering combinations; parameter accuracy was shown to be sensitive to the range of fiber diameters included in the analysis, but not to the number of intermediate fibers. Moreover, accurate parameter estimates were obtained without a priori knowledge about the spectral shape of γ. Observations were used to develop heuristic guidelines for the design of clinically applicable MDSFR probes.
Source: Biomedical Optics Express
DOI: 10.1364/boe.2.003150
Institution: Erasmus MC
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,913
OpenAlex ID: W2128689752