Work

Cyclic Caged Morpholinos: Conformationally Gated Probes of Embryonic Gene Function

Year: 2012

Type: article

Abstract: Feeling a bit cagey: morpholino-based antisense reagents have been caged through oligonucleotide cyclization, enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201201690

Authors: Sayumi Yamazoe, Ilya A. Shestopalov, Elayne Provost, Steven D. Leach, James K. Chen

Institutions: Stanford Medicine, Stanford University, Johns Hopkins University

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: Deciphering T-box gene-dependent mesoderm development with synthetic probes, Chemical Embryology: Technologies for Manipulating and Visualizing Development, Regulation of Exocrine Pancreatic Progenitors by Ptf1a-p48, Chemical Embryology: Technologies for Manipulating and Visualizing Development