Work

Fast wavelet-based single-particle reconstruction in Cryo-EM

Year: 2011

Type: conference-paper

Abstract: This paper presents a novel algorithm for the 3D tomographic inversion problem that arises in single-particle electron cryo-microscopy (Cryo-EM). It is based on two key components: 1) a variational formulation that promotes sparsity in the wavelet domain and 2) the Toeplitz structure of the combined projection/back-projection operator. The first idea has proven to be very effective for the recovery of piecewise-smooth signals, which is confirmed by our numerical experiments. The second idea allows for a computationally efficient implementation of the reconstruction procedure, using only one circulant convolution per iteration.

Source: IEEE International Symposium on Biomedical Imaging (ISBI)

DOI: 10.1109/isbi.2011.5872791

Authors: Cedric Vonesch, Lanhui Wang, Yoel Shkolnisky, Amit Singer

Institutions: Princeton University, Tel Aviv University

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: Improved Algorithms for Macromolecular Structure Determination by cyro-EM