Work

Transformation in Agmenellum quadruplicatum

Year: 1980

Type: article

Abstract: A DNA-mediated transformation system for the blue-green alga Agmenellum quadruplicatum, strain PR-6, is described and characterized for DNA concentration dependence, dependence on time of exposure to DNA, phenotypic expression, sensitivity to various enzymes, and competence. The stability of the transformants has been investigated, and genetic backcross and selfing experiments have been performed. This system fulfills all of the criteria established for the well-characterized transformation systems in heterotrophic bacteria and demonstrates significant similarities to at least one of these systems for all characteristics examined. The efficiency of transformation is high. This system fills a need for a well-characterized genetic system in an oxygen-evolving photoautotroph. We have used it to transform a strain with a mutational lesion in assimilatory nitrogen metabolism to a wild-type genotype.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.77.10.6052

Authors: S. Edward Stevens, Ronald D. Porter

Institution: Pennsylvania State University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: U.S. Department of Agriculture, U.S. Public Health Service

OpenAlex ID: W2129050237

FWCI: 3.505

Cites: 20

Cited by: 210

Related to: 10