Work

Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities

Year: 2011

Type: article

Abstract: Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.

Source: Environmental Microbiology Reports

DOI: 10.1111/j.1758-2229.2011.00275.x

Authors: Bernhard Knierim, Birgit Luef, Paul Wilmes, Richard I. Webb, Manfred Auer, Luis R. Comolli, Jillian F. Banfield

Institutions: Lawrence Berkeley National Laboratory, The University of Queensland, Centre for Microscopy and Microanalysis, University of California, Berkeley, University of Luxembourg, Luxembourg Centre for Systems Biomedicine, Centre de recherche public Gabriel-Lippmann

Language: English

Topic: Microbial Community Ecology and Physiology

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: green

Funders: U.S. Department of Energy, Alexander von Humboldt-Stiftung, Directorate for Biological Sciences, National Institute of General Medical Sciences, Biological and Environmental Research

Award: Electron Microscopy of Biological Macromolecules

OpenAlex ID: W2129308399

FWCI: 1.978

Cites: 19

Cited by: 20

Related to: 10