Expression of the Mitochondrial Genome in H E L a Cells
Year: 1973
Type: article
Abstract: The effect of selective inhibition of mitochondrial protein synthesis by chloramphenicol at 40 or 200 microg/ml on the formation of mitochondria in HeLa cells was investigated. HeLa cells, under the conditions used in the present work, grow at a decreasing rate for at least four cell generations in the presence of 40 microg/ml chloramphenicol, and for two generations in the presence of 200 microg/ml chloramphenicol. The progressive cell growth inhibition which begins after 2 days of exposure of the cells to 40 microg/ml chloramphenicol is immediately or gradually reversible, upon removal of the drug, for periods up to at least 8 days of treatment, though there is a progressive loss of cloning efficiency. In cells which have been treated for 6-7 days with 40 or 200 microg/ml of chloramphenicol, mitochondrial protein synthesis occurs at a normal or near-normal rate 1 h after removal of the drug. Mitochondria increase normally in number and show a normal size and amount of cristae in the presence of either concentration of drug. However, in 4-5% of the mitochondrial profiles the cristae appear to be arranged in unusual, circular, looped or whorled configuration.
Source: The Journal of Cell Biology
DOI: 10.1083/jcb.56.3.819
Authors: Brian Storrie, Giuseppe Attardi
Institution: California Institute of Technology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2129607679